Rapid astroglial stress response and elevation of endogenous BiP levels promote neuronal survival in hippocampal slice cultures

A hallmark of neurodegenerative diseases is the accumulation protein aggregates, formation which prevented by chaperone proteins. BiP central in endoplasmic reticulum. In this study we investigated pattern tunicamycin-stressed murine organotypic hippocampal slice cultures (OHCs). stressed OHCs highest apoptotic rates occur neurons CA1 regions and dentate gyrus, found levels to be lowest. Highest were astrocytes. Cell culture experiments indicated that stress response glial cells faster stronger than neuronal cells. We hypothesize rapid pronounced expression astrocytes helps maintain fine-balanced micromilieu necessary for survival neurons. SubAB a toxin, cleaves inactivates BiP. Low dosages did not elicit specific apoptosis was induced subfield. Mild prestressing with promoted viability tunicamycin-treated OHCs. conclude preconditioning tissue stressors elevate endogenous exert protective effect thereby promoting survival. These strengthen thesis preconditoning mild positively affects